General acquisition covering materials science and other use cases. For specialized techniques, use the appropriate subclass (AcquisitionSpa for single particle, or tomography subclasses).
Whether an energy filter was used and its specifics.
Number of grids imaged for this project - here with qualifier during this data acquisition
Time of data acquisition per movie/tilt - in s
Target defocus set, min and max values in nm.
Temperature during data collection, in K with min and max values.
Type of cryogen used in the holder - if the holder is cooled seperately
Level of binning on the images applied during data collection
Magnification level as indicated by the instrument, no unit
Number of images generated total for this data collection - might need a qualifier for tilt series to determine whether full series or individual tilts are counted
Machine estimated defocus, min and max values in µm. Has a tendency to be off.
Specifies which Acquisition subschema/class is in use.
Number of Beamtilt groups present in this dataset - for optimized processing split dataset into groups of same tilt angle. Despite its name Beamshift is often used to achive this result.
The size of the image in pixels, height and width given.
Number of frames that on average constitute a full movie, can be a bit hard to define for some detectors
Another way to move the beam above the sample for data collection purposes that does not require movement of the stage. Given in mrad.
Movement of the beam above the sample for data collection purposes that does not require movement of the stage. Given in mrad.
Whether and how you have to flip or rotate the gainref in order to align with your acquired images
Any type of special optics, such as a phaseplate
Cryogen used in cooling the instrument and sample, usually nitrogen
Average dose per image/movie/tilt - given in electrons per square Angstrom
The total electron beam current hitting the viewing screen, in nA.
Movement of the Beam below the image in order to shift the image on the detector. Given in µm.
List of custom descriptors for user-defined key-value pairs describing how micrographs were obtained or any related information
A series of numbers constructed from min, max, and increment
Minimal value of a given dataset property
Maximal value of a given dataset property
cryo electron tomography dataset of an environmental sample
Describe the instrument used to acquire the data
Describe the data acquisition parameters
The min, max and increment of the tilt angle in a tomography session. Unit is degree.
The tilt axis angle of a tomography series
if a value has a unit, it should be given as a unit value pair.
Special device used to correct instrument inherent chromatic aberration.
whether a specific instrument was used during data acquisition
Details of a given specialist instrument
Used to modulate the phase of the electron wave.
Special device used to correct instrument inherent spherical aberration.
humidity of the atmosphere right before/ during freezing; in %.
the cryogen used to freeze the sample, i.e. ethane
temperature of the sample right before freezing; in K.
Any further comments on the freezing process go here.
What was the atmosphere the sample was in right before freezing, elaborate on any special gases present etc.
freezing method - such as plunge freezing, high pressure freezing etc.
Unifying class to describe the full sample.
base information on the acquisition and treatment of the sample itself.
What was done to the sample, please give an overview of relevant treatments.
if the sample is a tissue sample please indicate what type of tissue.
the geographical location of your source, optimally in a geographic coordinate system.
where is this sample from? i.e. Hospital
the organism(s) present in your sample, if not perfectly defined try to asses as close as possible.
how the frozen sample was thinned by i.e. FiB-milling
The thinning method used, such as FIB milling.
What was the target thickness of the lamella; in nm.
what was the target of the tomograms, and what area of a cell do they cover.
If you have any further comments on your tomograms please leave them here
What was the main biological target of your research for this tomogram?
What type of cellular features are present in your tomograms?
Instrument values, mostly constant across a data collection.
Operating mode of the microscope, i.e. "TEM", "STEM"
Spherical aberration of the instrument, in mm
Voltage used for the electron acceleration, in kV
C2 aperture size used in data acquisition, in µm
Mode of imaging used during data collection
Type of electron source used in the microscope, such as FEG
Mode of illumination used during data collection
Refers to how tightly or widely the electron beam is focused onto the sample, in mrad. Typically low convergence for TEM and high for STEM.
Details on the person performing the experiment.
Role of the author, for example principal investigator
ORCID of the author, a type of unique identifier
Type of organization, academic, commercial, governmental, etc.
Overarching category for authors and grants
List of grants associated with the project
List of authors associated with the project
Any type, used as the base for type-narrowing.
Collection angle set, min and max values in mrad.
Mode of the detector, e.g. "counting", "ScanningDetector", "ImagingDetector", etc.
Dispersion of an analytical detector, in eV
A device used to filter for electrons with specific energy.
Optional optics used to correct for instrument limitations.
Specialist device to correct for chromatic aberration of the microscope lenses
Specialist device to correct for spherical aberration of the microscope lenses
Phaseplate is a special optics device that can be used to enhance contrast
The width of a given item - unit depends on item
The height of a given item - unit depends on item
an axis-aligned 2D bounding box (float units)
unit value extended to have two additional fields si_value and si_unit
value of a given field in respect to its SI unit