Average dose per image/movie/tilt - given in electrons per square Angstrom
Cryogen used in cooling the instrument and sample, usually nitrogen
Number of images generated total for this data collection - might need a qualifier for tilt series to determine whether full series or individual tilts are counted
The size of the image in pixels, height and width given.
Time of data acquisition per movie/tilt - in s
Movement of the beam above the sample for data collection purposes that does not require movement of the stage. Given in mrad.
Number of grids imaged for this project - here with qualifier during this data acquisition
Target defocus set, min and max values in nm.
Magnification level as indicated by the instrument, no unit
Another way to move the beam above the sample for data collection purposes that does not require movement of the stage. Given in mrad.
Whether and how you have to flip or rotate the gainref in order to align with your acquired images
Number of frames that on average constitute a full movie, can be a bit hard to define for some detectors
Temperature during data collection, in K with min and max values.
Movement of the Beam below the image in order to shift the image on the detector. Given in µm.
Whether an energy filter was used and its specifics.
The total electron beam current hitting the viewing screen, in nA.
Level of binning on the images applied during data collection
Number of Beamtilt groups present in this dataset - for optimized processing split dataset into groups of same tilt angle. Despite its name Beamshift is often used to achive this result.
Any type of special optics, such as a phaseplate
Type of cryogen used in the holder - if the holder is cooled seperately
Machine estimated defocus, min and max values in µm. Has a tendency to be off.
cryo electron tomography dataset of a cellular sample, lab grown
Describe the instrument used to acquire the data
Describe the data acquisition parameters
The min, max and increment of the tilt angle in a tomography session. Unit is degree.
The tilt axis angle of a tomography series
Special device used to correct instrument inherent chromatic aberration.
whether a specific instrument was used during data acquisition
Details of a given specialist instrument
Used to modulate the phase of the electron wave.
Special device used to correct instrument inherent spherical aberration.
were there any special treatment conditions; such as addition of certain chemicals etc
In/on what kind of surface/container the cells were grown; i.e. directly on a grid
What was the atmosphere the sample was in, elaborate on any special gases present etc.
What was the targeted cell cycle state, if any
Unifying class to describe the full sample with growth conditions
List of custom descriptors for user-defined key-value pairs describing how micrographs were obtained or any related information
A series of numbers constructed from min, max, and increment
Minimal value of a given dataset property
Maximal value of a given dataset property
Unifying class to describe the full sample.
Instrument values, mostly constant across a data collection.
Mode of imaging used during data collection
Operating mode of the microscope, i.e. "TEM", "STEM"
Type of electron source used in the microscope, such as FEG
Voltage used for the electron acceleration, in kV
Spherical aberration of the instrument, in mm
C2 aperture size used in data acquisition, in µm
Refers to how tightly or widely the electron beam is focused onto the sample, in mrad. Typically low convergence for TEM and high for STEM.
Mode of illumination used during data collection
Details on the person performing the experiment.
Role of the author, for example principal investigator
ORCID of the author, a type of unique identifier
Type of organization, academic, commercial, governmental, etc.
Overarching category for authors and grants
List of authors associated with the project
List of grants associated with the project
if a value has a unit, it should be given as a unit value pair.
General acquisition covering materials science and other use cases. For specialized techniques, use the appropriate subclass (AcquisitionSpa for single particle, or tomography subclasses).
Specifies which Acquisition subschema/class is in use.
What was the atmosphere the sample was in right before freezing, elaborate on any special gases present etc.
Any further comments on the freezing process go here.
the cryogen used to freeze the sample, i.e. ethane
humidity of the atmosphere right before/ during freezing; in %.
freezing method - such as plunge freezing, high pressure freezing etc.
temperature of the sample right before freezing; in K.
base information on the acquisition and treatment of the sample itself.
the geographical location of your source, optimally in a geographic coordinate system.
What was done to the sample, please give an overview of relevant treatments.
if the sample is a tissue sample please indicate what type of tissue.
where is this sample from? i.e. Hospital
the organism(s) present in your sample, if not perfectly defined try to asses as close as possible.
how the frozen sample was thinned by i.e. FiB-milling
The thinning method used, such as FIB milling.
What was the target thickness of the lamella; in nm.
what was the target of the tomograms, and what area of a cell do they cover.
What type of cellular features are present in your tomograms?
If you have any further comments on your tomograms please leave them here
What was the main biological target of your research for this tomogram?
Any type, used as the base for type-narrowing.
Dispersion of an analytical detector, in eV
Mode of the detector, e.g. "counting", "ScanningDetector", "ImagingDetector", etc.
Collection angle set, min and max values in mrad.
A device used to filter for electrons with specific energy.
Optional optics used to correct for instrument limitations.
Phaseplate is a special optics device that can be used to enhance contrast
Specialist device to correct for spherical aberration of the microscope lenses
Specialist device to correct for chromatic aberration of the microscope lenses
The width of a given item - unit depends on item
The height of a given item - unit depends on item
an axis-aligned 2D bounding box (float units)
unit value extended to have two additional fields si_value and si_unit
value of a given field in respect to its SI unit